Glutathione dosage guide

Glutathione dosage reference: typical range 200 – 600 mg, 2–3×/week (IV/IM) or daily (SubQ low-dose), cycle 8–12 weeks on, 2–4 weeks off. Titration chart…

The principal endogenous antioxidant — a tripeptide of glutamate, cysteine, and glycine. Central to detoxification, redox balance, and immune function; cellular levels decline with age and oxidative stress.

Published protocol literature for Glutathione describes a typical range of 200 – 600 mg, dosed 2–3×/week (iv/im) or daily (subq low-dose), over a cycle of 8–12 weeks on, 2–4 weeks off.

Glutathione dosage at a glance

ParameterReported value
Typical dose range200 – 600 mg
Frequency2–3×/week (IV/IM) or daily (SubQ low-dose)
Cycle length8–12 weeks on, 2–4 weeks off
RouteSubcutaneous injection (most published protocols)
DiluentBacteriostatic water
Commonly stacked withNAD+ (redox + energy), Vitamin C (regenerates GSH), Epitalon (longevity)

Glutathione titration chart

Protocols are usually titrated rather than started at the top of the range. The tiers below reflect how Glutathione dosing is commonly stepped in the published protocol literature.

TierDoseFrequencyNotes
Beginner200 mg2×/weekBaseline antioxidant support
Intermediate400 mg2–3×/weekDetox and skin-clarity protocols
Advanced600 mg3×/weekHigh oxidative load; clinical settings

Reconstitution and concentration

Concentration in mcg/mL equals vial milligrams multiplied by 1000, divided by the millilitres of bacteriostatic water added. Volume per dose in mL equals the target dose in mcg divided by that concentration. On a U-100 insulin syringe, multiply the volume in mL by 100 to read the units.

VialBacteriostatic waterResulting concentration
2 mg vial1 mL bacteriostatic water2,000 mcg/mL
5 mg vial2 mL bacteriostatic water2,500 mcg/mL
10 mg vial2 mL bacteriostatic water5,000 mcg/mL

Protocol notes

What Glutathione is studied for

Storage after reconstitution

StateConditionsShelf life
Lyophilized (Powder)Refrigerate 2–8°C (36–46°F) or freeze12–24 months refrigerated; longer frozen
ReconstitutedRefrigerate 2–8°C only; no freezing21–30 days typically
In TransitInsulated packaging; ice packsMinimize time; avoid heat exposure
MK-677 (Oral)Room temp okay; cool/dry preferredPer manufacturer; typically 12+ months

Sources

Important

Dose ranges on this page summarise published research and practitioner protocol literature for Glutathione. They are a reference, not medical direction. All peptide products referenced are intended solely for research, investigational, or licensed professional use. They are not FDA-approved and are not intended to diagnose, treat, cure, or prevent any disease. Consult a licensed medical practitioner before any personal or clinical use.

Frequently asked questions

What is a typical Glutathione dosage?

Published protocol literature for Glutathione generally describes 200 – 600 mg, administered 2–3×/week (iv/im) or daily (subq low-dose). Individual protocols vary and should be set by a licensed practitioner.

How often is Glutathione dosed?

2–3×/week (IV/IM) or daily (SubQ low-dose). Frequency is driven by half-life, so shorter-acting compounds are dosed more often.

How long is a Glutathione cycle?

Typical cycle length reported is 8–12 weeks on, 2–4 weeks off. Cycling exists to limit receptor desensitisation and to create assessment windows, not as a fixed rule.

How do you reconstitute Glutathione?

2mg → 1ml bacteriostatic water; 5mg → 2ml bacteriostatic water; 10mg → 2ml bacteriostatic water. Concentration in mcg/mL equals vial milligrams times 1000 divided by millilitres of bacteriostatic water.

Why is glutathione often discussed alongside NAD+?

Both are central to redox biology — NAD+ drives energy and repair pathways while glutathione handles antioxidant defense, so they appear together in longevity research.