Healing peptide protocols — BPC-157, TB-500, GHK-Cu. Reconstitution math, dose ranges, stacking, cycle length, and what the literature actually shows.
Recovery peptides are studied for soft-tissue and connective-tissue repair. BPC-157 and TB-500 are the two most referenced and are frequently run together, since they are described in the literature as acting through complementary pathways — angiogenesis and cell migration respectively — over 4–8 week cycles.
Category overview
Accelerate tissue repair, reduce inflammation, and rebuild stronger. Favorites among athletes, biohackers, and anyone recovering from injury or surgery.
Protocols at a glance
Peptide
Dose range
Frequency
Cycle
BPC-157
250 – 500 mcg
Daily to 2× daily
4–8 weeks acute; 2 weeks/month maintenance
TB-500
2 – 10 mg
1–2×/week
4-week loading + 4–8 week maintenance
BPC-157 / TB-500 Blend
0.25 – 0.5 mg of each peptide per dose (0.05 – 0.10 ml from a 2 ml reconstitution)
BPC-157 component daily or 2× daily; TB-500 loading 2×/week, then 1×/week maintenance — most users inject the blend daily during the 4-week loading phase, then taper TB-500 by reducing frequency
0.10 – 0.20 ml from a 4 ml reconstitution (≈0.25–0.5 mg each of TB-500, BPC-157, KPV + 1.25–2.5 mg GHK-Cu)
Daily to 5-on/2-off SubQ during an active repair or aesthetic phase; 2–3×/week for maintenance
8 weeks on, 4 weeks off
LL-37
50 – 400 mcg
Daily or every other day
4–8 weeks for acute issues; not for long-term continuous use
Pentadecapeptide
250 – 750 mcg
Daily or 2× daily
4–8 weeks on, 2–4 weeks off
BPC-157 — Body Protection Compound
Derived from a protective protein found in gastric juice. Legendary for healing tendons, ligaments, muscles, and gut. The most popular healing peptide.
BPC-157 / TB-500 Blend — 10 mg / 10 mg — "Wolverine Stack" in one vial
Pre-combined healing stack: 10 mg BPC-157 plus 10 mg TB-500 in a single vial. Pairs BPC-157's rapid tendon, ligament, and gut repair with TB-500's long-acting cell migration and anti-fibrotic effects — one injection, both pathways covered.
Dose range: 0.25 – 0.5 mg of each peptide per dose (0.05 – 0.10 ml from a 2 ml reconstitution). Frequency: BPC-157 component daily or 2× daily; TB-500 loading 2×/week, then 1×/week maintenance — most users inject the blend daily during the 4-week loading phase, then taper TB-500 by reducing frequency. Cycle: 4-week loading (daily) + 4–8 week maintenance (2–3×/week), then 2–4 week break.
Reconstitution: Total peptide per vial: 20 mg (10 mg BPC-157 + 10 mg TB-500)
Reconstitution: 20mg → 2ml bacteriostatic water · every 0.1 ml = 0.5 mg BPC-157 + 0.5 mg TB-500
Reconstitution: 20mg → 2.5ml bacteriostatic water · every 0.1 ml = 0.4 mg BPC-157 + 0.4 mg TB-500
Reconstitution: 20mg → 3ml bacteriostatic water · every 0.1 ml ≈ 0.33 mg of each (finer titration)
One-shot convenience — no separate BPC-157 and TB-500 vials to manage
Synergistic tendon, ligament, and muscle repair (the classic "Wolverine Stack")
BPC-157 drives fast localized healing and gut repair
TB-500 promotes cell migration, flexibility, and scar-tissue reduction systemically
Both peptides are SubQ-compatible; inject near the injury site when feasible for BPC-157 effect, otherwise rotate abdomen/thigh sites.
Refrigerate after reconstitution; use within 30 days. Protect from light.
If you only need acute tendon/gut repair, plain BPC-157 may be more cost-effective. The blend is best when you want both immediate (BPC) and long-acting systemic (TB-500) recovery.
Naturally occurring tripeptide with copper binding. Multi-functional for wound healing, collagen synthesis, and anti-aging. Available injectable, topical, and in skincare.
Dose range: 0.5 – 3 mg. Frequency: Daily or split doses. Cycle: 8 weeks on, 4 weeks off (injectable).
Reconstitution: 2mg → 1ml bacteriostatic water
Reconstitution: 5mg → 2ml bacteriostatic water
Reconstitution: 10mg → 2ml bacteriostatic water
Accelerates wound healing via copper-mediated signaling
Boosts collagen and elastin for skin/joint health
Potent antioxidant protecting against oxidative damage
Hair follicle regeneration and hair growth support
Four-peptide aesthetic and repair blend in a single vial. TB-500 and BPC-157 cover systemic and localized tissue repair, GHK-Cu drives collagen and skin renewal, and KPV — the non-pigmenting anti-inflammatory fragment of α-MSH — calms the inflammatory environment around healing tissue.
Dose range: 0.10 – 0.20 ml from a 4 ml reconstitution (≈0.25–0.5 mg each of TB-500, BPC-157, KPV + 1.25–2.5 mg GHK-Cu). Frequency: Daily to 5-on/2-off SubQ during an active repair or aesthetic phase; 2–3×/week for maintenance. Cycle: 8 weeks on, 4 weeks off.
Next-generation variant of BPC-157 with modified amino acid sequence for potentially enhanced stability and efficacy.
Dose range: 250 – 750 mcg. Frequency: Daily or 2× daily. Cycle: 4–8 weeks on, 2–4 weeks off.
Reconstitution: 2mg → 1ml bacteriostatic water
Reconstitution: 5mg → 1ml bacteriostatic water
Reconstitution: 10mg → 2ml bacteriostatic water
Enhanced stability compared to standard BPC-157
Similar healing profile: tendons, ligaments, gut, systemic
Potentially longer half-life for sustained effects
Reduced injection frequency possible
Emerging compound; conservative approach.
Stacks: TB-500, GHK-Cu, standard BPC-157 (rotation)
Safety principles
Start Low, Go Slow: Always begin with the minimum effective dose and titrate gradually over weeks.
Get Baseline Bloodwork: Before starting, obtain labs including CBC, CMP, lipid panel, hormone panel (testosterone, estrogen, IGF-1, GH), fasting insulin, HbA1c, liver enzymes, and inflammatory markers.
Monitor Regularly: Retest labs every 4–8 weeks. Track subjective markers daily (sleep, energy, mood, appetite, injection site reactions).
Source Responsibly: Only use peptides from reputable compounding vendors with third-party COA testing. We STRONGLY recommend they also are based out of the USA for quality assurance.
Recognize Warning Signs: Stop immediately and seek medical attention for severe injection site reactions, difficulty breathing, chest pain, severe headache, vision changes, or allergic reactions.
Drug Interactions: Many peptides interact with insulin, diabetes drugs, blood thinners, immunosuppressants, and hormonal therapies.
Contraindications: Not for pregnant/breastfeeding women, individuals under 18, those with active cancer, severe liver/kidney disease, or uncontrolled diabetes.
Important
All peptide products referenced are intended solely for research, investigational, or licensed professional use. They are not FDA-approved and are not intended to diagnose, treat, cure, or prevent any disease. Consult a licensed medical practitioner before any personal or clinical use.